From: casi casi Date: Wed Jan 24, 2001 10:08pm Subject: Re: phenolic exudations Hello World! These exudates are usually considered to the various phenolic compounds that oxidize to form brown material in the medium. These substances tend to be inhibitory to development. Treatments to minimize this problem include pretreting the explants whith an antioxidant (citric or ascorbic acid) including an adsorbent material in the medium (polyvinilpyrrolidone or activated charcol) You can use Gerlite instead of Agar. It is not clear if Gerlite prevents the exudation or the oxidation of that exudation. Jorge Guzman