Von: TQPLlab@awinthrop.freeserve.co.uk [mailto:TQPLlab@awinthrop.freeserve.co.uk] Gesendet am: Donnerstag, 19. Juli 2001 20:59 An: Ederer Thomas Betreff: paph germination Hi Thomas, Good that you have some Paphiopedilum and Phragmipedium seed. Generally Paph seed is not so prolific in germination compared to other orchids, though there are always exceptions. Primary hybrids (species x species) often produce good germination and large numbers. Some species also germinate abundantly. Phragmipediums are very variable in their germination response and are very much controlled by the genetics and ploidy (chromosome number). Generally it is belived that dark is required for germination for Paphs and Phrags. Light can definately inhibit germination in some Paph seed sowings, however both of these can sometimes germinate in the light and this may lead to quicker germination and or growth. It is impossible to predict what does what unless you know your breeding lines very well. The best practical approach is to put most of your flasks into the dark, and perhaps keep 1/10 in the light after sowing. You watch your Paph and Phrag seed carefully, and after 6 weeks look for germination and swelling of seed. When you think 50% have germinated as white protocorms (in the dark) or when the seedlings become drawn upwards - move them to the very low light and gradually increase this over a few weeks. The protocorms will become green. Germination may continue over a long period. Warning do not use banana added to media for sowing / germinating Paphs as it stops growth! Some Paph seed does not germinate for at least 9 months or much longer... so never give up. Then you will see leaves develop. If they are maudiae type, make sure you replate soon as the roots become easily tangled. There are some pictures of the germintion process on the website under the laboratory section. Perhaps let me know how things progress Best regards and good flasking. Alan TQPLlab@awinthrop.freeserve.co.uk http://www.tissuequickplantlabs.com