From: Damon and JaNaye Bryson Date: Tue Jan 9, 2001 8:39pm Subject: Re: your response on Catlya culture We use a liquid media called C2. Ingredients as follows: Sucrose 20g/l Citric acid 0.150 g/l A. MgSO4 - 7H2O 0.4g/l MnS04-H2O 0.0075g/l B. KCl 0.5 g/l KH2PO4 0.25 g/l D. FeSO4 0.01067 g/l EDTA 0.0224 g/l E. IBA 0.00175 g/l F. NAA 0.00175 g/l G. Ca(NO3)-H2O 1.0 g/l H. (NH4)2SO4 0.4 g/l I. Thiamine 0.0001 g/l Nicotinic Acid 0.0005 g/l Pyridoxine 0.0005 g/l Glycine 0.002 g/l J. H3B03 0.0015 g/l ZnSO4-7H2O 0.0015 g/l CuS04-5H2O 0.00005 g/l The letters above correspond to ingredients in a single stock solution. To make stock solutions, multiply the grams above by 100 and add 500ml distilled water. Freeze half of solution and use and refrigerate other half. Always check stock solutions for contamination before using, if contaminated, defrost and use frozen portion. When making medium, use 5 ml of each stock solution per liter. If solid medium is required, use 8 g/l agar. After making medium, aprox. 5 ml is placed in large test tubes and autoclaved for 30 min. and 200 psi. Cultures are initiated on an inclined, rotating wheel at 5 rpm; 28 degrees C, and 12 hours photo period under cool white flourescents. We use this medium for initiation of all our clones including cats, dendrobiums, cymbidiums, bulbophylums, brassias, vandas, etc. Hope this helps. JaNaye